Biochemistry

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Molecular Glues: The Adhesive Connecting Targeted Protein Degradation to the Clinic	65
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Cysimiditides: RiPPs with a Zn-Tetracysteine Motif and Aspartimidylation	48
A High-Throughput Screening Pipeline to Identify Methyltransferase and Exonuclease Inhibitors of SARS-CoV-2 NSP14	44
Structure–Function Analysis of Streptomyces griseolus CYP105A1 in the Metabolism of Nonsteroidal Anti-inflammatory Drugs	41
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Identifying Distinct Structural Features of the SARS-CoV-2 Spike Protein Fusion Domain Essential for Membrane Interaction	32
Computational Approaches for Development of Engineered Therapeutics against SARS-CoV-2	32
Biosynthetic Strategies of Berberine Bridge Enzyme-like Flavoprotein Oxidases toward Structural Diversification in Natural Product Biosynthesis	32
Introduction to the Protein Condensates Virtual Special Issue	32
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A Specific pSer/Thr-Pro Motif Generates Interdomain Communication Bifurcations of Two Modes of Pin1 in Solution Nuclear Magnetic Resonance	31
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Assembling a Robust Workflow for Characterizing Endogenous E3 Ligase Substrates	27
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Fragment-Based Discovery of Novel VE-PTP Inhibitors Using Orthogonal Biophysical Techniques	26
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Molecular Insights into the Inhibition of Dialysis-Related β2m Amyloidosis Orchestrated by a Bispidine Peptidomimetic Analogue	25
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PLP-Dependent Enzyme Methionine γ-Lyase: Insights into the Michaelis Complex from Molecular Dynamics and Free Energy Simulations	24
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Novel Mechanisms of Molecular Glue-Induced Protein Degradation	23
Molecular Glues: A New Approach to Modulating GPCR Signaling Bias	23
The Huntingtin Transport Complex	23
Protein and DNA Conformational Changes Contribute to Specificity of Cre Recombinase	23
Regulation of GLP-1 and Glucagon Receptor Function by β-Arrestins in Metabolically Important Cell Types	22
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Parameterizing the Binding Properties of XNA Aptamers Isolated from a Low Stringency Selection	21
Development of Peptide Displacement Assays to Screen for Antagonists of DDB1 Interactions	21
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A Novel Color Switch of Microbial Rhodopsin	20
Structural and Biochemical Characterization of the Human Angiogenin–Proliferating Cell Nuclear Antigen Interaction	20
Human Translesion Synthesis Polymerases polκ and polη Perform Error-Free Replication across N2-dG Methyleugenol and Estragole DNA Adducts	20
Biochemical and HDX Mass Spectral Characterization of the SARS-CoV-2 Nsp1 Protein	20
RNA Polymerase Ribozyme That Recognizes the Template–Primer Complex through Tertiary Interactions	20
A Single DNA Point Mutation Leads to the Formation of a Cysteine–Tyrosine Crosslink in the Cysteine Dioxygenase from Bacillus subtilis	20
Engineering a Red Fluorescent Protein Chromophore for Visualization of RNA G-Quadruplexes	20
Rapid Kinetics of Pistol Ribozyme: Insights into Limits to RNA Catalysis	20
Conformation-Dependent Hydrogen-Bonding Interactions in a Switchable Artificial Metalloprotein	18
Slow-Onset, Potent Inhibition of Mandelate Racemase by 2-Formylphenylboronic Acid. An Unexpected Adduct Clasps the Catalytic Machinery	18
How to Kinetically Dissect an RNA Machine	18
Complex Formation of Heme Oxygenase-2 with Heme Is Competitively Inhibited by the Cytosolic Domain of Caveolin-1	18
Phase Separation in Cell Polarity	18
A Streamlined Data Analysis Pipeline for the Identification of Sites of Citrullination	18

Liquid–Liquid Phase Separation in Biology: Specific Stoichiometric Molecular Interactions vs Promiscuous Interactions Mediated by Disordered Sequences	18
The Salmonella Effector SspH2 Facilitates Spatially Selective Ubiquitination of NOD1 to Enhance Inflammatory Signaling	18
Common Pitfalls and Recommendations for Using a Turbidity Assay to Study Protein Phase Separation	18
VPS26 Moonlights as a β-Arrestin-like Adapter for a 7-Transmembrane RGS Protein in Arabidopsis thaliana	17
Photosynthetic Growth and Energy Conversion in an Engineered Phototroph Containing Thermochromatium tepidum Light-Harvesting Complex 1 and the Rhodobacter sphaeroides Reaction Center Com	17
CG17192 is a Phospholipase That Regulates Signaling Lipids in the Drosophila Gut upon Infection	17
Inactivation of CYP2D6 by Berberrubine and the Chemical Mechanism	17
Differential Interactions of Selected Phytocannabinoids with Human CYP2D6 Polymorphisms	17
Water’s Variable Role in Protein Stability Uncovered by Liquid-Observed Vapor Exchange NMR	16
Characterization of a Nitro-Forming Enzyme Involved in Fosfazinomycin Biosynthesis	16
Mechanism of Catalysis and Substrate Binding of Epoxyqueuosine Reductase in the Biosynthetic Pathway to Queuosine-Modified tRNA	16
Opposing Effects of PhoPQ and PmrAB on the Properties of Salmonella enterica serovar Typhimurium: Implications on Resistance to Antimicrobial Peptides	16
How Salt and Temperature Drive Reentrant Condensation of Aβ40	16
Beyond the Plateau: pL Dependence of Proton Inventories as a Tool for Studying Ribozyme and Ribonuclease Catalysis	16
Factor XIII Activation Peptide Residues Play Important Roles in Stability, Activation, and Transglutaminase Activity	16
Site-Specific Steric Control of SARS-CoV-2 Spike Glycosylation	16
Epoxyqueuosine Reductase QueH in the Biosynthetic Pathway to tRNA Queuosine Is a Unique Metalloenzyme	15
Discovery of an Allosteric, Inactive Conformation-Selective Inhibitor of Full-Length HPK1 Utilizing a Kinase Cascade Assay	15
Molecular Insights into Conformational Heterogeneity and Enhanced Structural Integrity of Helicobacter pylori DNA Binding Protein Hup at Low pH	15
Natural and Synthetic Suppressor Mutations Defy Stability–Activity Tradeoffs	15
Peptide-Dependent Growth in Yeast via Fine-Tuned Peptide/GPCR-Activated Essential Gene Expression	15
Second-Shell Amino Acid R266 Helps Determine N-Succinylamino Acid Racemase Reaction Specificity in Promiscuous N-Succinylamino Acid Racemase/o-Succinylbenzoate Synthase Enzymes	15
Protein Engineering in Ribosomally Synthesized and Post-translationally Modified Peptides (RiPPs)	15
Structural Insights into Molecular Recognition by Human Chemokine CCL19	15
Molecular Characterization of Ancient Prosaposin-like Proteins from the Protist Dictyostelium discoideum	15
Three Rings to Rule Them All: How Versatile Flavoenzymes Orchestrate the Structural Diversification of Natural Products	15
Designed Trp-Cage Proteins with Antimicrobial Activity and Enhanced Stability	15
Effects of Familial Alzheimer’s Disease Mutations on the Assembly of a β-Hairpin Peptide Derived from Aβ16–36	15
Structural and Thermodynamic Model for the Activation of Cardiac Troponin	14
X-ray Crystal Structures of Methane Monooxygenase Hydroxylase Complexes with Variants of Its Regulatory Component: Correlations with Altered Reaction Cycle Dynamics	14
Evidence for Proline Catabolic Enzymes in the Metabolism of Thiazolidine Carboxylates	14
Bacterial Responses to Iron Withholding by Calprotectin	14
Resolving the Hydride Transfer Pathway in Oxidative Conversion of Proline to Pyrrole	14
Hysteresis in Hepatitis B Virus (HBV) Requires Assembly of Near-Perfect Capsids	14
Accumulation and Pulse Electron Paramagnetic Resonance Spectroscopic Investigation of the 4-Oxidobenzyl Radical Generated in the RadicalS-Adenosyl-l-methionine Enzyme HydG	14
Multiple Domains of Staphylococcal Superantigen-like Protein 11 (SSL11) Contribute to Neutrophil Inhibition	14
Effects of Stromal and Lumenal Side Perturbations on the Redox Potential of the Primary Quinone Electron Acceptor QA in Photosystem II	14
High-Throughput Algorithmic Optimization of In Vitro Transcription for SARS-CoV-2 mRNA Vaccine Production	14
Signal Transmission in Escherichia coli Cyclic AMP Receptor Protein for Survival in Extreme Acidic Conditions	14
Kinetic, Inhibition, and Structural Characterization of a Malonate Semialdehyde Decarboxylase-like Protein from Calothrix sp. PCC 6303: A Gateway to the non-Pro1 Tautomerase Superfamily Members	14
Glycerol 3-Phosphate Dehydrogenase Catalyzed Hydride Transfer: Enzyme Activation by Cofactor Pieces	14
Insights into Binding of Single-Stranded Viral RNA Template to the Replication–Transcription Complex of SARS-CoV-2 for the Priming Reaction from Molecular Dynamics Simulations	14
Conditional Alternative Protein Splicing Promoted by Inteins from Haloquadratum walsbyi	14
Metal Dependence and Functional Diversity of Type I Cas3 Nucleases	14
Modification of the 4Fe-4S Cluster Charge Transport Pathway Alters RNA Synthesis by Yeast DNA Primase	13
p62 Phase-Separation as the Foundation of Autophagy-Based Degraders	13
Covalent Adduct Formation in Methylthio-d-ribose-1-phosphate Isomerase: Reaction Intermediate or Artifact?	13
Interaction of the Influenza A Virus NS1 Protein with the 5′-m7G-mRNA·eIF4E·eIF4G1 Complex	13
A Closed Form Model for Molecular Ratchet-Type Chemically Induced Dimerization Modules	13
Structure and Dynamics of the Flexible Cardiac Troponin T Linker Domain in a Fully Reconstituted Thin Filament	13
Targeting Scaffolding Functions of Enzymes Using PROTAC Approaches	13
Genome Engineering for Next-Generation Cellular Immunotherapies	13
Metabolic Processing of Selenium-Based Bioisosteres of meso-Diaminopimelic Acid in Live Bacteria	13
Principles Governing the Phase Separation of Multidomain Proteins	13
Structural Insights into Binding of Remdesivir Triphosphate within the Replication–Transcription Complex of SARS-CoV-2	13
Cysteine Dealkylation in Bacillus subtilis by a Novel Flavin-Dependent Monooxygenase	13
PARP1 Incises Abasic Sites and Covalently Cross-links to 3′-DNA Termini via Cysteine Addition Not Reductive Amination	12
Effect of Phosphate and Ferritin Subunit Composition on the Kinetics, Structure, and Reactivity of the Iron Core in Human Homo- and Heteropolymer Ferritins	12
Structure-Guided Insight into the Specificity and Mechanism of a Parasitic Nucleoside Hydrolase	12
Combined Pulsed Electron Double Resonance EPR and Molecular Dynamics Investigations of Calmodulin Suggest Effects of Crowding Agents on Protein Structures	12
Vitamin E Does Not Disturb Polyunsaturated Fatty Acid Lipid Domains	12
Small Changes Make the Difference for SIRT2: Two Different Binding Modes for 3-Arylmercapto-Acylated Lysine Derivatives	12
Structures of Plasmodium falciparum Chloroquine Resistance Transporter (PfCRT) Isoforms and Their Interactions with Chloroquine	12
Identification and Characterization of the 28-N-Methyltransferase Involved in HSAF Analogue Biosynthesis	12
Identification of Filamin A Mechanobinding Partner III: SAV1 Specifically Interacts with Filamin A Mechanosensitive Domain 21	12
A Direct Assay for Measuring the Activity and Inhibition of Coactivator-Associated Arginine Methyltransferase 1	12
Bioinspired Design of Artificial Signaling Systems	12
The Metabolome Weakens RNA Thermodynamic Stability and Strengthens RNA Chemical Stability	12
A Novel Gene Alignment in Dorea sp. AM58-8 Produces 7-Dehydroxy-3β Bile Acids from Primary Bile Acids	12
DJ-1 Molecular Chaperone Activity Depresses Tau Aggregation Propensity through Interaction with Monomers	12
Synthesis of Bispecific Antibody Conjugates Using Functionalized Poly-ADP-ribose Polymers	12
Predicted Structure of the BciC Enzyme Catalyzing the Removal of the C132-Methoxycarbonyl Group for Biosynthesis of Chlorosomal Chlorophylls: A Mechanism for Dual Catalytic Functions of Hyd	12
The PqsE Active Site as a Target for Small Molecule Antimicrobial Agents against Pseudomonas aeruginosa	12
C3- and C3/C5-Epimerases Required for the Biosynthesis of the Capsular Polysaccharides from Campylobacter jejuni	12
Transient Prenyltransferase–Cyclase Association in Fusicoccadiene Synthase, an Assembly-Line Terpene Synthase	12
Ganglioside-Enriched Phospholipid Vesicles Induce Cooperative Aβ Oligomerization and Membrane Disruption	12
Using a Single Peptide to Electrochemically Sense Multiple Kinases	12
Transient-State Kinetic Analysis of the RNA Polymerase II Nucleotide Incorporation Mechanism	11
Identification of Novel Compounds Inhibiting the Kinase Activity of the CDK5/p25 Complex via Direct Binding to p25	11
Contrasting Mechanisms of Aromatic and Aryl-Methyl Substituent Hydroxylation by the Rieske Monooxygenase Salicylate 5-Hydroxylase	11

Role of the Coiled-Coil Domain in Allosteric Activity Regulation in Soluble Guanylate Cyclase	11
Lipidome of the Bacteroides Genus Containing New Peptidolipid and Sphingolipid Families Revealed by Multiple-Stage Mass Spectrometry	11
13C Electron Nuclear Double Resonance Spectroscopy-Guided Molecular Dynamics Computations Reveal the Structure of the Enzyme–Substrate Complex of an Active, N-Linked Glycosylated Lip	11
Cyanobacterial Phycobilisome Allostery as Revealed by Quantitative Mass Spectrometry	11
Inside-Out Design of Zinc-Binding Proteins with Non-Native Backbones	11
Discovery and Characterization of Antibody Probes of Module 2 of the 6-Deoxyerythronolide B Synthase	11
Structure-Based Optimization of Protease–Inhibitor Interactions to Enhance Specificity of Human Stefin-A against Falcipain-2 from the Plasmodium falciparum 3D7 Strain	11
Trypanosoma brucei Mitochondrial DNA Polymerase POLIB Contains a Novel Polymerase Domain Insertion That Confers Dominant Exonuclease Activity	11
Mechanism of Asparagine-Mediated Proton Transfer in Photosynthetic Reaction Centers	11
Putative Protein Interactome of the Rhomboid Protease RHBDL4	11
Elucidation of Short Linear Motif-Based Interactions of the FERM Domains of Ezrin, Radixin, Moesin, and Merlin	11
Structure-Based Engineering of a Sesquiterpene Cyclase to Generate an Alcohol Product: Conversion of epi-Isozizaene Synthase into α-Bisabolol Synthase	10
Oligomeric Changes Regulate Flavin Transfer in Two-Component FMN Reductases Involved in Sulfur Metabolism	10
The Long Road to a Synthetic Self-Replicating Central Dogma	10
Optimizing the First TPR Domain of the Human SPAG1 Protein Provides Insight into the HSP70 and HSP90 Binding Properties	10
Observation of pH-Dependent Residual Structure in the Pmel17 Repeat Domain and the Implication for Its Amyloid Formation	10
Annexins A1 and A2 Accumulate and Are Immobilized at Cross-Linked Membrane–Membrane Interfaces	10
DNA Sequence Control of Enzyme Filamentation and Activation of the SgrAI Endonuclease	10
Tryptophan-Centric Bioinformatics Identifies New Lasso Peptide Modifications	10
Development of Enzyme-Based Approaches for Recycling PET on an Industrial Scale	10
The Burden Borne by Protein Methyltransferases: Rates and Equilibria of Non-enzymatic Methylation of Amino Acid Side Chains by SAM in Water	10
Structure-Guided Discovery of N5-CAIR Mutase Inhibitors	10
Structural Characterization of 5-Substituted Pyrrolo[3,2-d]pyrimidine Antifolate Inhibitors in Complex with Human Serine Hydroxymethyl Transferase 2	10
BRD4354 Is a Potent Covalent Inhibitor against the SARS-CoV-2 Main Protease	10
The Impact of Second-Shell Nucleotides on Ligand Specificity in Cyclic Dinucleotide Riboswitches	10
Discrete Acyltransferases and Thioesterases in Iso-Migrastatin and Lactimidomycin Biosynthesis	10
Initial Steps in Methanobactin Biosynthesis: Substrate Binding by the Mixed-Valent Diiron Enzyme MbnBC	10
Characterization of the Membrane-Associated Electron-Bifurcating Flavoenzyme EtfABCX from the Hyperthermophilic Bacterium Thermotoga maritima	10
Correction to “β-Hairpin Alignment Alters Oligomer Formation in Aβ-Derived Peptides”	10
Conformational Morphing by a DNA Analogue Featuring 7-Deazapurines and 5-Halogenpyrimidines and the Origins of Adenine-Tract Geometry	10
Probing the Diacylglycerol Binding Site of Presynaptic Munc13-1	10
Electrostatic Interactions Dictate Bile Salt Hydrolase Substrate Preference	10
Characterization of a FourU RNA Thermometer in the 5′ Untranslated Region of Autolysin Gene blyA in the Bacillus subtilis 168 Prophage SPβ	10
Lipid-Binding Regions within PKC-Related Serine/Threonine Protein Kinase N1 (PKN1) Required for Its Regulation	10
Buffers, Especially the Good Kind	10
Distinguishing Different Hydrogen-Bonded Helices in Proteins by Efficient 1H-Detected Three-Dimensional Solid-State NMR	10
A Stutter in the Coiled-Coil Domain of Escherichia coli Co-chaperone GrpE Connects Structure with Function	10
Decreased Transition-State Analogue Affinity in Isotopically Heavy MTAN with Increased Catalysis	10
Isolation and Partial Characterization of Novel, Structurally Uniform (Hfq6)n≥8 Assemblies Carrying Accessory Transcription and Translation Factors	9
Modifying the Basicity of the dNTP Leaving Group Modulates Precatalytic Conformational Changes of DNA Polymerase β	9
N-Terminal Extensions Appear to Frustrate HU Heterodimer Formation by Strengthening Intersubunit Contacts and Blocking the Formation of a Heterotetrameric Intermediate	9
Anticancer Drugs of Lysine Specific Histone Demethylase-1 (LSD1) Display Variable Inhibition on Nucleosome Substrates	9
Cryo-EM Structure of the Photosynthetic LH1-RC Complex from Rhodospirillum rubrum	9
How ATP and dATP Act as Molecular Switches to Regulate Enzymatic Activity in the Prototypical Bacterial Class Ia Ribonucleotide Reductase	9
Structurally Based Design of Glucagon Mutants That Inhibit Fibril Formation	9
Active-Site Oxygen Accessibility and Catalytic Loop Dynamics of Plant Aromatic Amino Acid Decarboxylases from Molecular Simulations	9
Crystal Structure of the Carbohydrate Recognition Domain of the Human Macrophage Galactose C-Type Lectin Bound to GalNAc and the Tumor-Associated Tn Antigen	9
Structural Basis for the Interactions of the Colibactin Resistance Gene Product ClbS with DNA	9
An Unusual Ferryl Intermediate and Its Implications for the Mechanism of Oxacyclization by the Loline-Producing Iron(II)- and 2-Oxoglutarate-Dependent Oxygenase, LolO	9
A Complete Survey of RhoGDI Targets Reveals Novel Interactions with Atypical Small GTPases	9
Distinguishing Concerted versus Stepwise Mechanisms Using Isotope Effects on Isotope Effects	9
Cryo-EM Structure of the Human Amylin 1 Receptor in Complex with CGRP and Gs Protein	9
Axial Heme Coordination by the Tyr-His Motif in the Extracellular Hemophore HasAp Is Critical for the Release of Heme to the HasR Receptor of Pseudomonas aeruginosa	9
Unraveling the Structure-Spectrum Relationship of Yeast Phenylalanine Transfer RNA: Insights from Theoretical Modeling of Infrared Spectroscopy	9
Direct Mapping of Higher-Order RNA Interactions by SHAPE-JuMP	9
Probing the Catalytic Mechanism and Inhibition of SAMHD1 Using the Differential Properties of Rp- and Sp-dNTPαS Diastereomers	9
Mechanisms of O2 Activation by Mononuclear Non-Heme Iron Enzymes	9
The Crystal Structure of Calmodulin Bound to the Cardiac Ryanodine Receptor (RyR2) at Residues Phe4246–Val4271 Reveals a Fifth Calcium Binding Site	9
Structural Insights of a cis-Epoxysuccinate Hydrolase Facilitate the Development of Robust Biocatalysts for the Production of l-(+)-Tartrate	9
Exploring the Conformational Landscape of a Lanthipeptide Synthetase Using Native Mass Spectrometry	9
Biochemical, Bioinformatic, and Structural Comparisons of Transketolases and Position of Human Transketolase in the Enzyme Evolution	9
Elucidating the Role of Human ALAS2 C-terminal Mutations Resulting in Loss of Function and Disease	9
Correlated Motions in Structural Biology	9
Poly-GR Impairs PRMT1-Mediated Arginine Methylation of Disease-Linked RNA-Binding Proteins by Acting as a Substrate Sink	9
Structural Dynamics of Cytochrome P450 3A4 in the Presence of Substrates and Cytochrome P450 Reductase	9
Inhibitory Effects, Fluorescence Studies, and Molecular Docking Analysis of Some Novel Pyridine-Based Compounds on Mushroom Tyrosinase	9
EPR Spin-Trapping for Monitoring Temporal Dynamics of Singlet Oxygen during Photoprotection in Photosynthesis	9
The 2-Aminopyridine Nucleobase Improves Triple-Helical Recognition of RNA and DNA When Used Instead of Pseudoisocytosine in Peptide Nucleic Acids	9
From the Three-Dimensional Structure of Phosphotriesterase	8
Substrate Specificity of a Methyltransferase Involved in the Biosynthesis of the Lantibiotic Cacaoidin	8
Pif1 Helicase Mediates Remodeling of Protein-Nucleic Acid Complexes by Promoting Dissociation of Sub1 from G-Quadruplex DNA and Cdc13 from G-Rich Single-Stranded DNA	8
Lipid Nanoparticles Deliver mRNA to the Brain after an Intracerebral Injection	8
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Effects of Ca2+ on the Structure and Dynamics of PIP3 in Model Membranes Containing PC and PS	8
Discovery of Cryptic Natural Products Using High-Throughput Elicitor Screening on Agar Media	8
Fine-Tuning of ATF4 DNA Binding Activity by a Secondary Basic Motif Unique to the ATF-X Subfamily of bZip Transcription Factors	8
The Structure of Carbamoylphosphate Synthetase Unravels Central Functional Features of a Key Metabolic Multienzyme Complex	8
RNA Post-transcriptional Modifications of an Early-Stage Large-Subunit Ribosomal Intermediate	8
RNA Post-Transcriptional Modifications in Two Large Subunit Intermediates Populated in E. coli Cells Expressing Helicase Inactive R331A DbpA	8
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Elucidating the Mechanism of Recognition and Binding of Heparin to Amyloid Fibrils of Serum Amyloid A	8
A Major Disease-Related Point Mutation in Spastin Dramatically Alters the Dynamics and Allostery of the Motor	8
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Induction of Paraptotic Cell Death in Cancer Cells by Triptycene–Peptide Hybrids and the Revised Mechanism of Paraptosis II	8
Delivery Aspects for Implementing siRNA Therapeutics for Blood Diseases	8
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Modulating α-Synuclein Liquid–Liquid Phase Separation	8
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The Thermal-Stable LH1–RC Complex of a Hot Spring Purple Bacterium Powers Photosynthesis with Extremely Low-Energy Near-Infrared Light	8
Presenilin, γ-Secretase, and the Search for Pathogenic Triggers of Alzheimer’s Disease	8